ApartSIL C18 HS is a type of high-performance liquid chromatography (HPLC) column
that is designed for reversed-phase chromatography. It is commonly used in the separation
and analysis of various compounds, especially those that are non-polar or moderately polar.
Below is an overview of the characteristics, applications, and technical details of the
ApartSIL C18 HS column.
1. General Characteristics
Type: Reversed-phase HPLC column.
Stationary Phase: Silica particles coated with C18 (Octadecyl) groups.
Particle Size: Typically available in sizes like 3µm, 5µm, etc.
Pore Size: Often around 100 Å (angstroms), which is suitable for a wide range of
molecular weights.
Surface Area: High surface area for better interaction with analytes.
2. Functional Groups
C18 (Octadecyl) Chains: The C18 chains provide hydrophobic interactions, which
are crucial for retaining and separating non-polar compounds. The "HS" in the name
likely stands for "High Surface" or "High Strength," indicating enhanced surface
interaction or durability of the stationary phase.
3. Applications
Pharmaceutical Analysis: Commonly used for the separation and quantification of
drug compounds and impurities.
Environmental Testing: Utilized in the analysis of pollutants, pesticides, and organic
contaminants in water, soil, and air samples.
Food and Beverage Testing: Employed to analyze additives, contaminants, and
nutritional components.
Biochemical Analysis: Used for the separation of peptides, proteins, and other
biomolecules.
4. Operating Conditions
pH Range: Typically stable between pH 2 and 8. However, the actual pH range can
vary depending on the specific product variant.
Temperature Range: The column can generally operate between ambient
temperature and up to 60-80°C, depending on the specific manufacturer guidelines.
Mobile Phases: Compatible with a wide range of organic solvents like methanol,
acetonitrile, and aqueous buffers.
5. Performance
Efficiency: High efficiency due to the small particle size and large surface area,
resulting in sharp peaks and good resolution.
Selectivity: Offers excellent selectivity for non-polar to moderately polar compounds.
Load Capacity: Suitable for both analytical and preparative separations, depending
on the column dimensions.
6. Maintenance and Care
Column Conditioning: Requires proper conditioning with compatible solvents
before first use.
Storage: Should be stored in a solvent like methanol or acetonitrile when not in use to
prevent drying out and degradation.
Cleaning: Periodic flushing with strong solvents may be required to remove strongly
retained contaminants.
7. Variants
ApartSIL columns may come in different pore sizes, particle sizes, and column
dimensions (length and internal diameter) to cater to specific separation needs.
There may be other versions of ApartSIL columns with different functional groups for
various applications, such as C8, phenyl, or cyano phases.
8. Manufacturers and Suppliers
The ApartSIL brand may be associated with specific manufacturers specializing in
HPLC columns. The exact specifications might vary slightly depending on the
manufacturer, so it’s essential to consult the specific product datasheet.
9. Technical Support and Documentation
Manufacturers typically provide detailed user manuals and technical datasheets that
offer guidelines on column usage, recommended mobile phases, pH stability, and
cleaning procedures.
10. Typical Applications in Research and Industry
Pharmaceutical QC: For routine quality control and assay of active pharmaceutical
ingredients (APIs).
Environmental Labs: For trace analysis of organic pollutants.
Academic Research: For method development and compound characterization.
Overall, the ApartSIL C18 HS column is a versatile and widely used tool in analytical
chemistry, providing reliable and high-quality separations for a broad range of compounds.