ApartSIL C30 India

ApartSIL C30 is an HPLC (High-Performance Liquid Chromatography) column designed for reversed-phase chromatography, featuring a C30 (octadecyl) stationary phase bonded to a silica support. This column type provides unique separation characteristics due to its high hydrophobicity and long carbon chain. Here’s a comprehensive overview:

General Characteristics

Type:Reversed-phase HPLC column.
Stationary Phase: Silica particles bonded with C30 (octadecyl) groups.
C30 Designation: Refers to the presence of a long chain of 30 carbon atoms (octadecyl groups) attached to the silica surface, resulting in very high hydrophobic interactions.

Particle and Pore Characteristics

Particle Size: Available in various particle sizes such as 3µm, 5µm, or 10µm. Smaller particles generally provide higher resolution and efficiency.
Pore Size:Typically around 100 Å (angstroms), suitable for separating small to moderately large molecules.
Surface Area: High surface area due to finely divided silica particles, enhancing interaction between analytes and the stationary phase.

Functional Groups and Bonding

Octadecyl Groups: The column features octadecyl groups (C30) bonded to the silica surface. These long carbon chains provide extremely high hydrophobic interactions with analytes.
Bonding Technology: The C30 groups are bonded using advanced silanization techniques, ensuring stability and reproducibility of the column’s performance.

Applications

Pharmaceutical Analysis: Effective for separating very hydrophobic pharmaceuticals, including drugs with high lipophilicity. Suitable for complex pharmaceutical formulations and drug discovery.
Environmental Testing: Useful for analyzing hydrophobic environmental pollutants, pesticides, and organic contaminants.
Food and Beverage Analysis: Suitable for separating food additives, contaminants, and other highly hydrophobic organic compounds in food and beverages.
Biochemical Analysis: Employed for separating large biomolecules and complex mixtures with high hydrophobicity.
Clinical Research: Effective for analyzing biological samples, including lipophilic drugs and metabolites.

Performance and Stability

High Hydrophobic Interaction: The C30 groups provide very strong hydrophobic interactions, making the column ideal for separating highly non-polar compounds.
High Efficiency: Offers excellent separation efficiency with well-resolved peaks, which is essential for accurate quantification and identification of complex samples.
Reproducibility: Ensures consistent and reproducible results, which is crucial for routine analyses and method validation.

Operating Conditions

pH Range:Typically stable across a pH range of 2 to 8, allowing flexibility in mobile phase composition. Caution should be exercised at extreme pH levels.
Temperature Range: Generally stable up to 60-80°C, depending on the specific column specifications.
Mobile Phases: Compatible with a variety of organic solvents (e.g., methanol, acetonitrile) and aqueous buffers. Mobile phase composition can be optimized for specific applications.

Maintenance and Care

Column Conditioning: Proper conditioning with recommended solvents before initial use is necessary to achieve optimal performance.
Cleaning Protocols: Regular cleaning with appropriate solvents (e.g., methanol, acetonitrile) helps maintain performance by removing retained contaminants.
Storage: Should be stored in a suitable solvent (such as methanol or acetonitrile) to prevent the stationary phase from drying out and to avoid microbial growth.

Advantages of the C30 Phase

Extreme Hydrophobic Interaction: The C30 groups provide the highest level of hydrophobic interactions among reversed-phase columns, making it suitable for very hydrophobic analytes.
Excellent Separation Efficiency: Offers high separation efficiency with well-resolved peaks, crucial for analyzing complex samples.
Versatility in Complex Mixtures: Effective in separating complex mixtures where other reversed-phase columns may not perform as well.

Variants and Customization

Different Particle and Pore Sizes: Available in various configurations to suit specific analytical needs, including different particle sizes and pore sizes.
Column Dimensions: Offered in different lengths (e.g., 50mm, 100mm, 150mm, 250mm) and internal diameters (e.g., 2.1mm, 4.6mm) to cater to different applications, from high-throughput analysis to preparative chromatography.

Technical Specifications

Silica Support: Made from high-purity silica, minimizing metal contamination that could interfere with separation.
Endcapping: Often endcapped to reduce the activity of residual silanol groups, improving peak shape and reducing tailing for basic compounds.
Bonding Process: The C30 groups are bonded using advanced silanization techniques, ensuring stable and consistent performance.

Applications in Research and Industry

Pharmaceutical Quality Control: Used in quality control labs for analyzing highly lipophilic pharmaceuticals and their metabolites.
Environmental Analysis: Effective for analyzing very hydrophobic pollutants and contaminants.
Food Safety Testing: Useful for analyzing food products for highly hydrophobic additives and contaminants.
Chemical Manufacturing: Employed in monitoring chemical processes and purification steps where high hydrophobicity is involved.
Clinical and Biomedical Research: Facilitates the separation and identification of highly hydrophobic organic compounds, aiding in drug development and disease research.

Method Development

Optimization Flexibility: The column’s high hydrophobicity allows for flexibility in method development, particularly for complex and highly hydrophobic analytes.
Compatibility with Gradient Elution: Performs well under both gradient and isocratic elution conditions, providing versatility in method design.

Regulatory Compliance

Pharmaceutical and Food Testing: Often used in methods that comply with regulatory standards (e.g., USP, EP, FDA), making it suitable for validated analytical procedures.

Manufacturers and Suppliers

Availability:ApartSIL C30 columns are produced by various chromatography product manufacturers. Specific details may vary, so consulting the product datasheet is recommended.
Technical Support: Manufacturers typically provide comprehensive technical support, including user guides, troubleshooting advice, and method development assistance.

Comparative Advantages

High Hydrophobic Interaction Benefits: Provides effective separation for highly hydrophobic compounds due to the extreme hydrophobic interactions of the C30 groups.
Excellent for Complex Samples: Suitable for complex mixtures where other reversed-phase columns may not offer sufficient resolution.

Interaction Benefits

Enhanced Separation: The long carbon chains facilitate strong hydrophobic interactions with non-polar analytes, improving separation efficiency and peak shape.
High Purity: Ensures minimal contamination and reliable separation results, which is critical for accurate analytical work.

style="text-align:justify;"In summary, ApartSIL C30 is an HPLC column designed for reversed-phase chromatography with C30 (octadecyl) functional groups bonded to the silica surface. Its extreme hydrophobicity makes it ideal for separating very hydrophobic compounds, including complex mixtures and pharmaceuticals. The column offers excellent separation efficiency, versatility, and reproducibility, making it a valuable tool for diverse analytical applications in pharmaceuticals, environmental analysis, food safety, and biochemical research.