The C8 HPLC column is more suitable for analyzing macromolecular substances, such as some globulins, etc., which are used in conjunction with C8 and a buffer salt eluent
C8 is recommended for separation on highly hydrophobic compounds and LC/MS instruments. C8 requires less analysis time than C18 when neutralizing neutral or other compounds with strong retention under the same chromatographic conditions.
Providing robust performance for analysis of a wide variety of compounds, C18 has been used in pharmaceuticals, materials, food, environmental, manufacturing, and other industries. C18 columns constitute a mainstay in many analytical chemists' inventory for testing.
This HPLC column is widely used for basic compounds. The alcohol-based site can effectively prevent the interaction between the alkaloid and the silanol group, and the alkaline substance has a good peak shape.
RP-8 /18 is a highly versatile reversed-phase HPLC column suitable for acidic, neutral and weakly basic compounds. Its optimal selectivity and performance ensure reproducible separations from batch to batch.
A cyano column can be used when a highly hydrophobic compound cannot be eluted with a standard C18 and C8 column or a typical reversed-phase eluent. The normal phase column can replace the silica gel column, which has a fast equilibrium and is more consistent than the activity of the non-derivatized silica gel.
Cyano HPLC column can be used for normal phase and reverse phase separation;
When used in the reverse phase, it has different selectivity from C18 universal HPLC column and C8 universal HPLC column.